The pH of the medium
was adjusted to 5.7 using 1 N KOH or HCl, prior to autoclaving at
1.05 kg cm−2 pressure at 121 ◦C for 20 min. The cultures were maintained
at 25
±
2◦ C under 16 h photoperiod with light intensity of
35–40 mol m−2 s−1 (cool white fluorescent tubes). All micropropagated
plantlets, from their respective experiments, were of the
same age and genetically uniform.