Nine-year-old buffalo at full term of pregnancy was presented to the PAU clinics with the complaints of dystocia, anuria and constipation. Surgery was performed to relieve dystocia, but as the condition of the animal deteriorated after surgery, the animal was euthanized. Necropsy revealed thickening of the wall of the urinary bladder which was hard to cut and rough with few irregular papilliform projections in the lumen. Microscopic examination indicated thickening of wall of the urinary bladder due to urothelial hyperplasia and hypertrophy. Neoplastic transitional epithelial cells were seen infiltrating the lamina propria and occasionally to the tunica muscularis of the bladder. Marked lymphoid cell infiltration was observed in between the infiltrating tumour cells and also in the form of well-defined multifocal perivascular aggregates. No significant histopathological findings were detected in regional and distant lymph nodes and other visceral organs, thus, ruling out the possibility of metastasis.