The current method allows the simultaneous determination of nicotine and its 10 major metabolites
(metabolite coverage about 95% of the absorbed dose) from a small sample volume and within a reasonable amount of time. Due to its wide dynamic range, high sensitivity and high throughput capabilities,
this method could serve as a powerful tool for quantifying the nicotine dose of smokers, passive smokers
as well as novel tobacco and nicotine product users in clinical and epidemiological studies.