Different annealing temperatures were evaluated for each pair of designed primers. The PCR mixture (20 L) contained 100 ng DNA template, 10 L 2× Master Mix (Microanalysis, Pearland, TX, USA) and 500 nmol/L each primer.
Different annealing temperatures were evaluated for each pair of designed primers. The PCR mixture (20 L) contained 100 ng DNA template, 10 L 2× Master Mix (Microanalysis, Pearland, TX,USA) and 500 nmol/L each primer.