Yeast count and percentage viability were determined by a modified methylene blue staining method on a Thoma chamber (Erten, 1997). Samples for esters and higher alcohols analysis were centrifuged and supernatants were kept at 20 C until analyzed.
Yeast count and percentageviability were determined by a modified methylene blue stainingmethod on a Thoma chamber (Erten, 1997). Samples for estersand higher alcohols analysis were centrifuged and supernatantswere kept at 20 C until analyzed.