Within the n-3 PUFA family, the LSO diet increased the proportions of 18:3n-3 (P,0·001) and 20:5n-3 (P¼0·002), but it had no effect on 22:5n-3 and 22:6n-3 proportions (Table 2). Within the n-6 PUFA family, the proportion of 18:2n-6 was greater (P¼0·006), while the proportion of 20:4n-6 was lesser (P¼0·03) in the LSO group than in the LAR group (Table 2).