Following purification, the purity and molecular weights of AKR
and SDRwere assessed by SDS-PAGE (resolving gel,10% acrylamide/
bis-acrylamide, pH 8.8; and stacking gel, 5% acrylamide/bisacrylamide, pH 6.8). Electrophoresis was carried out with a constant voltage of 130 V. After electrophoresis, gels were stained by
immersing in Coomassie Blue R250 solution at room temperature
for 60min. Excess stainwas removed by destainingwith solution of
methanol (45% v/v) and acetic acid (10% v/v).