Our early elutions for the quantitative determination of c-oryzanol revealed
also some experimental drawbacks in the RP-HPLC methods. In
fact, apolar stationary phases are highly retentive of triacylglycerides
(TAG), free fatty acids (FFA) and waxes. A proper column rinsing,
before re-equilibration, demands to change from water
miscible to water immiscible eluents.