The volume was raised
to 10 ml with distilled water. The sample was covered with one
drop of mineral oil. The PCR reaction was initiated by denaturation
at 94 8C for 3 min followed by 35 cycles of 94 8C for 30 s, 55 8C for
30 s and 72 8C for 1 min and 30 s. A final 5 min incubation at 72 8C
was allowed for the completion of primer extension