The vitellogenin concentrations in whole-body homogenates of juvenile zebrafish
exposed to metazachlor-based herbicide Butisan 400 SC and 17β-oestradiol were measured
at 40 dph. In the whole-body homogenates of fish from the negative control group, the
median vitellogenin concentration was 616 ng·ml-1. Medians of vitellogenin concentrations
in whole-body homogenates of fish exposed to Butisan 400 SC at concentrations of 0.1,
1.0 and 5.0 mg·l-1 were 916; 556 and 1254 ng·ml-1, respectively. The median value of
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vitellogenin concentration in whole-body homogenates of fish exposed to 17β-oestradiol
at a concentration of 100 ng·l-1 was 10 562 ng·ml-1. (Fig. 1 and Fig. 2).
Among the groups tested, significant differences were found (Kruskal–Wallis test: Q =
33.68; N = 60; p < 0.001). Multiple comparison of the mean ranks confirmed a significant
(p < 0.05) increase in vitellogenin concentration in fish exposed to 17β-oestradiol
compared to fish from the control group and groups exposed to 0.1 mg·l-1 or 1.0 mg·l-1 of
the preparation tested (see Fig. 1).