To be considered as positive, a signal generated in the CoSYPS Path Food detection system should display an
(exponential) amplification above the limit of detection of each qPCR determined previously, with the expected Tm-value (Barbau-Piednoir et al., 2013a, 2013b). The combination of positive assays generates the list of bacteria possibly present into the sample (presumptive positive) according to the decision tree presented in Fig. 2.