A factorial experiment was arranged in a randomized complete block design with three replications. Explants used in the experiment were 2 g of PLBs for Brand’s bottles, 3 g of PLBs for Kedaung’sbottles and 100-ml Erlenmeyer flasks, 7.5 g of PLBs for 250-mLErlenmeyer flasks and 10 g PLBs/500 ml medium for the bioreactor.PLBs were subcultured every month for four months.