Microbial and chemical properties may change depending on the culture media used and the country where the grain is produced. Silva et al. (2009) indicated that sugary kefir grains fermented with brown sugar show the greatest antimicrobial activity and inhibit Candida albicans, Salmonella typhi, Shigella sonnei, Staphylococcus aureus and Escherichia coli when compared to kefir fermented using molasses and demerara sugar. The interactions between yeasts and LAB also significantly change depending on the sugar source ( Leroi and Pidoux, 1993b). However, few studies have reported on the effect of culture media on the microbial profile of sugary kefir and its grains.