After isolation, these organisms were grown on Caldo L (composition: 10 g tryptone, 5 g yeast extract and 3 g NaCl in 1 L of distilled water) and were given a heat shock until sporulation
After isolation, these organisms were grown on Caldo L(composition: 10 g tryptone, 5 g yeast extract and 3 g NaCl in 1 L of distilledwater) and were given a heat shock until sporulation