Nitrogen balance also
highlights the existence of a somewhat extended denitrification
process, although no final anoxic phase was included in
the treatment cycle. In fact, less than 1 mg/l of TKN was
required for biomass growth, considering a nitrogen request
of about 4.5 mgN/gCODremoved, calculated by multiplying
sludge production (0.15 gTSS/gCODremoved; see paragraph 3.2)
and measured N biomass content (0.03 gN/gTSS).