. The PCR reactions were carried out in 25 μL volumes on an Applied
Biosystems 2720 Thermocycler (Applied Biosystems Life Technologies,
Grand Island, NY, USA), with the following conditions: 94 °C
4 min; 94 °C 40 s, 55 °C 40 s, 72 °C 1 min, total 30 cycles; 72 °C
10 min, hold at 4 °C.