. Leuconostoc
mesenteroides (KCTC 13302) and Lactobacillus sakei (KCTC
13416) were purchased from Korea Biological Resource Center, as a
starter culture. Leu. medenteriodes and Lb. sakei were sub-cultured
twice in deMan Rogasa Sharpes (MRS) broth at 30 °C and 37 °C for
2 days, respectively. The 10 mL of the suspension was centrifuged
at 3000 g (4 °C) for 15 min, and then collected cells were washed
in a sterile 0.85% NaCl solution twice. The number of microbes was
adjusted to 1 107 CFU/mL and inoculated to kimchi samples. The
ripening and fermentation of kimchi was carried out for 24 hours
using an incubator (IB-600M, JEIO TECH, Korea) at 30 °C. Kimchi
samples were collected every three hours after incubation and
immediately measured for their pH and total acidity. All experiments
were performed under standard aseptic condition.