Experimental design
Each trial involved ten females (either all wild type or all red,
depending on the trial) and two males, one wild type and one red. The
males in each trial were closely size-matched (mean difference in
length=2.2%; weight=5.8%) with body weight of at least 9% more
than the mean weight of the females. Females were transferred into
the experimental tank a day earlier than the males. Each trial ran for
5 weeks, during which the fish were left to spawn naturally in the
arena tank. Standard length and weight of all adult fish were taken
before and after every trial.
A total of eight trials were carried out (wild type females in four
trials, red in the other four), alternating between trials involving wild
type and red females. Different males were used for every trial, from a
variety of different families and avoiding repeating a pairing of males
from the same families (additionally, males were not taken from the
same families as females used in these trials). Each set of ten females
(and replacements from the same source if necessary) was used for
two trials.
Experimental design
Each trial involved ten females (either all wild type or all red,
depending on the trial) and two males, one wild type and one red. The
males in each trial were closely size-matched (mean difference in
length=2.2%; weight=5.8%) with body weight of at least 9% more
than the mean weight of the females. Females were transferred into
the experimental tank a day earlier than the males. Each trial ran for
5 weeks, during which the fish were left to spawn naturally in the
arena tank. Standard length and weight of all adult fish were taken
before and after every trial.
A total of eight trials were carried out (wild type females in four
trials, red in the other four), alternating between trials involving wild
type and red females. Different males were used for every trial, from a
variety of different families and avoiding repeating a pairing of males
from the same families (additionally, males were not taken from the
same families as females used in these trials). Each set of ten females
(and replacements from the same source if necessary) was used for
two trials.
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