Only a single study exists for B. tomentosa
(Naz et al. 2012). Nodal explants from in vitro seedlings
could form 12 shoots per explant after 6 weeks
on MS medium containing 50 mg·dm-3
adenine
sulphate (AS), 5.0 µM BA and 0.5 µM NAA. Roots
were induced in the presence of 5.0 µM chlorogenic
acid and 2.5 µM IBA from 70% of shoots within 4
weeks. MS was more effective than WPM as a basal
medium. Polyvinylpyrrolidone and ascorbic acid
were able to reduce polyphenolics in vitro.
B. vahlii cotyledonary nodes from 15-d old
in-vitro-germinated seedlings could form 5.5 shoots
per explant on MS medium containing 20 g·dm-3
sucrose, 1 μM TDZ, at 40 μmol·m
-2
·s
-1
PPFD under
a 16-h photoperiod (Upreti and Dhar 1996). This
medium served equally well for shoot induction
and proliferation. MS as basal medium was more
effective than B5 (Gamborg et al. 1968), WPM or
½ MS. Some success in inducing shoots was possible
with 1 μM BA, 1 μM Kin, or 2.5 μM zeatin.