2.5. Extraction and determination of total phenolics content, total flavonoid content and antioxidant activity
The samples were extracted using the method adopted from Abu Bakar, Mohamed, Rahmat, and Fry (2009). The extracts prepared from the freeze-dried fruits (1 g) were extracted for 2 h with 10 ml of 80% methanol at room temperature on an orbital shaker set at 180 rpm. The mixture was centrifuged at 1400 g for 20 min and the supernatant was decanted into a 30 ml vial. The pellet was re-extracted under identical conditions. The supernatant was combined and used for the total antioxidant activity, total phenolics and total flavonoid contents.