The enzymatic hydrolysis of SA-AWB revealed that, when a tem-perature lower than or equal to 40◦C was used for pretreatment,glucose yields ranging from 11.0 to 48.9% were observed even after96 h of hydrolysis (Table 4). Glucose predominated in all substratehydrolysates, with cellobiose always corresponding to less than 2%of the total glucose equivalents measured by HPLC. This meansthat, under the conditions used in this study, Cellic®CTec3 hadenough -glucosidase activity to avoid the end-product inhibitionthat is associated with cellobiose accumulation. A relatively lowhydrolysis yield was also observed for sample SA-AWB11. Hence,the use of intermediate temperatures of 55◦C was not enough toovercome the lack of reagent that in this case corresponded to thelowest NaOH loading applied in the entire experimental design(0.0824 g g−1NaOH). On the other hand, at temperatures above orequal to 70◦C, the hydrolysis yields were between 77.2 and 96.9%in 96 h. The best hydrolysis performance was achieved with sam-ple SA-AWB6 (0.125 g g−1NaOH; 70◦C; 140 W). Sample SA-AWB12also reached a high glucose yield after enzymatic hydrolysis (78.9%)and this was attributed to the use of a NaOH concentration of0.2926 g g−1at an intermediate reaction temperature.