Conventional methods like colony morphology, staining techniques and various biochemical tests including carbohydrate disc fermentation tests (Himedia, India) were adopted for bacterial identification.
A definite chromogenic method for Bacillus identification was done by growing the bacterial cultures in HiCrome Bacillus agar (Himedia, India).
16S rRNA-encoding gene amplification and sequencing was used as a confirmatory tool for bacterial species identification.