Plaques produced by each individual virus (on
nutrient plates) were examined for plaque morphology and measured (in mm).
Individual viruses were then examined by transmission electron microscopy
(TEM). For TEM, a mesh copper grid with carbon-coated Formvar film was
floated on a droplet of the phage sample and then stained with 2% uranyl
acetate. Images (15,500 and 28,500 magnifications) were collected using a
Philips CM-100 TEM operated at 80 kV, spot 3, 200-m condenser aperture, and
50-m objective aperture.
Plaques produced by each individual virus (onnutrient plates) were examined for plaque morphology and measured (in mm).Individual viruses were then examined by transmission electron microscopy(TEM). For TEM, a mesh copper grid with carbon-coated Formvar film wasfloated on a droplet of the phage sample and then stained with 2% uranylacetate. Images (15,500 and 28,500 magnifications) were collected using aPhilips CM-100 TEM operated at 80 kV, spot 3, 200-m condenser aperture, and50-m objective aperture.
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