The enzyme carbonic anhydrase is one of the fastest enzymes that catalyzes the hydration of CO2 bicarbonate HCO3
ð Þ and protons (H+), with hydration turn over number around 106 s
1. The catalytic efficiency of this enzyme is dependent on the proton shuttle which is nearly the diffusion limit in buffers with pKa >8. Hence this is the ideal enzyme for CO2 capture because of its fastest kinetics as well as its potential to identify enzymes that catalyzes the reaction under varied pH and temperature conditions