To examine the changes in the chemical compositions
of cell wall during the hot-pressing process, lignin and
holocellulose content of the residue samples were determined.
Klason lignin and acid-soluble lignin were measured
by the Klason method.11 In this procedure, a dry sample of
1.0 g was used; the secondary hydrolysis with 3% sulfuric
acid was carried out at 121°C for 30min. Holocellulose
content was determined as the NaClO2-delignified residue:
samples (2.5 g) were repeatedly (four times) treated with 2g
of NaClO2 in dilute acetic acid aqueous solution (150 ml) at
75°C for 1h.12
To examine t