The phytochemical screening of rhizome of aqueous extract of Alpinia calcarata and Alpinia galanga was performed using standardprocedures. The total phenolic and flavonoid content were determined by Folin-Ciocalteau and Aluminium chloride reagents. The variousantioxidant assays and cytotoxic assays (MTT) for Alpinia calcarata and Alpinia galanga was performed using standard methods (DPPH radicalscavenging assay, Nitric oxide radical scavenging assay, Reducing power assay, Phosphomolybdenum reduction assay).