Rotarod assay, a simple and accurate approach to examining agerelated
changes in balance and motor coordination (Baur et al., 2006),
was performed using the rotarod apparatus (Med Associates, St. Albans
VT) at 14 and 25 month old mice to examine their ability to remain on
an revolving rod (Coyle et al., 2008). Briefly, amouse was briefly placed
in a separate lane of a rotarod with an accelerating speed (2–20 rpm)
until the mouse fell off the rod. The length of time and speed of the
mouse which stayed on the rod were recorded.