Mesophilic, total coliforms, Bacillus and moulds and yeasts counts were significantly reduced after the HPTP that demonstrated the effectiveness of this kind of treatment on the native microorganisms of pumpkin. Thus the thermal impact may become a determining factor for the HPTP at certain conditions, which include the initial temperature or matrix composition, it makes important to improve understanding of the effects of HPTP on health-related compounds. Other important attributes like color may be affected, increasing CIE L* values. HPTP of pumpkin purée at moderate temperatures may be a suitable alternative to preserve and even increase the bioavailability of health-related compounds of pumpkin purée since the levels of carotenoids, phenolics acidsor antioxidant compounds were increased (better extracted)after processing. The combination of 600 MPa with an initial temperature of 70◦C achieved greater inactivation of polyphenoloxidase enzyme, increased levels of bioactive compounds(carotenoids and polyphenols) and the highest antioxidant activity of processed pumpkin purée. The resistance of this enzyme to HPTP emphasizes the necessity of combining this treatment with other techniques, such as storage at low temperature or addition of inhibitors to maintain food quality and extend shelf-life. In addition, since intensity of the treatments applied should not reach a sterilization effect, storage at low temperatures should be recommended after processing.