Starch preparation
Rice kernels were soaked in water for 4 h and then ground with water using a double disc stone mill. The slurry was centrifuged for 15min.
and dried at 45∘C for 15 h. The flour was ground in a hammer mill and passed through a 100-mesh sieve; then it was stored at room temperature in sealed plastic bags. The isolation of waxy rice flour was mixed sodium hydroxide solution , it was stirred filtered and centrifuged. The sediment was washed with water, neutralized with hydrochloric acid solution, and dried.