Abstract: Collection of soil and plant samples from nine different geographical locations in Uttar Pradesh state of
India was made. Composite soil was analyzed for its nutrient status. A total of sixty isolates of Fusarium species
were recovered from the soil and plant samples. Among these, thirty nine isolates were identified as Fusarium
oxysporum on the basis of their morphological and molecular identification. The pathogenicity test was conducted
on tomato variety Pant T-3, disease incidence ranged from zero to 78.74%. Among F. oxysporum isolates, five were
non pathogenic and three were found strongly pathogenic.Non-pathogenic isolates were tested for their antagonistic
effect against most pathogenic isolates of F. oxysporum. The results showed that the Isolate no. 40 showed the
highest antagonistic activity in inhibiting radial growth of pathogenic isolates.