Fig. 4. Structural features of native PV2 from P. canaliculata. (A) Absorption spectra of PV2 (solid line), with the fourth derivative spectra (dashed line). (B) Tryptophan fluorescence
spectrum of PV2. (C) CD spectra of PV2 in the near-UV region (dashed line) and far-UV region (solid line). (D) Dissociating gel electrophoresis (SDS-PAGE) was performed in 4–20%
w/v polyacrylamide gradient. Lane 1: Low molecular weight standards; Lane 2: PV2 incubated with β-mercaptoethanol; Lane 3: PV2 without reducing agent