We isolated volatile metabolite-producing endophytic fungi from the leaves of the rubber tree (Hevea brasiliensis Müll.Arg.) and studied their antimicrobial competence. A total of three isolates was obtained, and their phenotypic and phylogenetic relationship with the genus Muscodor in the family Xylariaceae was studied. All isolates could produce volatile metabolites with apparent antimicrobial activity against diverse test microbes (bacteria, yeast and filamentous fungi). An isolate, RTM5IV3, with