The methodology was designed to be performed easily,
rapidly, and relatively cheaply, with restriction enzymes
selected to generate distinct differences between RFLP
profiles, allowing fish species to be uniquely classified.
This was so that many samples could easily be identified
by comparison with authentic species. The only drawback
of the method for differentiating between species
is the inability to distinguish between two closely
related hake species. However, further work to identify
an enzyme capable of differentiating these two species
could be performed if required.