Real-time monitoring of amplification by the RT-LAMP assay
The real-time monitoring of amplification of the dengue RT-LAMP assay was observed with
Loopamp real-time turbidimeter (LA-320, Teramecs, Co., Ltd., Japan). The reaction is considered positive when the turbidity reached 0.1 within 60 min at 650 nm. The time needed for the turbidity of each tested sample to exceed at 0.1 is referred to as the threshold time (Tt) [7].