RESULTS AND DISCUSSION
During this study, 100 samples of liver and intestine were investigated, after receiving the obtained samples,
immediately they were cultured at Tripticase Soy Agar (TSA). Gram staining, catalase and oxidase tests were used
for confirming primary isolated bacteria samples. Then 50 samples from suspected strains (Gram negative , catalase
positive and oxidase negative ) were isolated for molecular diagnosis and detection by PCR method, of which 3
cases were positive . After PCR, the 573 bp fragment obtained bands, were blasted with other sequences
associated with Y. ruckeri in the gene bank.
This study was carried out for molecular detection of Y. ruckeri bacterium in farmed rainbow trout cultured in
western part of Iran. Results led to identification of these bacteria in the aforementioned fishes. This bacterium is the
infectious agent of farmed fishes and the cause of high losses and mortality in different species of fishes. With
regard to the growth of aquaculture in Iran, infectious diseases are also frequently reported in rainbow trout farms in
recent years. Prevalence of Streptococcosis disease in Chaharmahal va Bakhtiari province, is one of the cases. Identifying and controlling of infectious diseases, should be paid
RESULTS AND DISCUSSION
During this study, 100 samples of liver and intestine were investigated, after receiving the obtained samples,
immediately they were cultured at Tripticase Soy Agar (TSA). Gram staining, catalase and oxidase tests were used
for confirming primary isolated bacteria samples. Then 50 samples from suspected strains (Gram negative , catalase
positive and oxidase negative ) were isolated for molecular diagnosis and detection by PCR method, of which 3
cases were positive . After PCR, the 573 bp fragment obtained bands, were blasted with other sequences
associated with Y. ruckeri in the gene bank.
This study was carried out for molecular detection of Y. ruckeri bacterium in farmed rainbow trout cultured in
western part of Iran. Results led to identification of these bacteria in the aforementioned fishes. This bacterium is the
infectious agent of farmed fishes and the cause of high losses and mortality in different species of fishes. With
regard to the growth of aquaculture in Iran, infectious diseases are also frequently reported in rainbow trout farms in
recent years. Prevalence of Streptococcosis disease in Chaharmahal va Bakhtiari province, is one of the cases. Identifying and controlling of infectious diseases, should be paid
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