Methods: The gastric acid secretion of isolated mouse whole stomach was stimulated by histamine (5.0 μM) or
bethanechol (10 or 100 μM) after adding the inhibitors (atropine 1 μM, ranitidine 10 μM, indomethacin 0.1 μM or
L-NAME 300 μM) and/or Ya-hom to the serosal solution. The effluent perfusate was collected continuously in
10-minute fractions for 120 minutes after stimulation.