on in vitro basal GtH-II release and pituitary responsiveness to GnRH were examined using common carp pituitary fragments in a perifusion system. Salmon GnRH superagonist [A&, Pro’ NEtI-sGnRH (sGnRH-A) was administered in pulsatile and continuous manners under different temperature conditions. The results demonstrate that temperature influences both the short and longer term GtH-II release responses to sGnRH-A. Institute, La Jolla, CA), was diluted with HBSS from stock solution (10 PM in HBSS) immediately before use. t influences of temperature