All methods except those of Englyst [22,49,53] for
NSP include lignin. In the enzymatic-gravimetric
methods, lignin is included as part of the residue
after filtration. In the enzymatic-chemical methods
of Theander and coworkers [54] and Southgate
[45], lignin is analyzed as a separate component,
using the Klason lignin method [55]. This method
measures native lignin, but can also include
tannins, cutins, and Maillard reaction products