The primers used for RT and amplification of CMV RNA were oligonucleotide primers (CMV-1 (5 GCC GTA AGC TGG ATG GAC AA 3) and CMV-2 (5 TAG GAT AAG CTT GTT TCG CG 3) specific for a 485 nucleotide sequence (EU428827) corresponding to 3 end coat protein and C-terminal non-coding region of RNA-3.