Lactobacillus Isolation
Initially, 120 microbial colonies were obtained from
human feces as raw material. Thirty colonies were selected
based on criteria such as morphological shape, catalysis
negativity, Gram positivity, and lactic acid formation,
and they were considered to be LAB. Among these 30
colonies, five strains (L. plantarum CK 101, CK 102,
CK 103, CK 104, and CK 105) were found to form
clear round zones in solid MRS medium containing 0.5%
bile salt. The clear zones around the colonies showed
hydrolysis reaction by bile salt dehydrogenase, and their
size indicated proportionality of bile salt dehydrogenase
activity. These five strains were selected and kept for the
next experiment.