The explants were placed in culture vessels containing 20 mls of culture media containing MS basal salts supplemented with 20 g/l sucrose, vitamins; glycine 2 g/l, pyridoxine 0.5 g/l, Nicotinic acid 0.5 g/l, Thiamine 0.1 g/l and Myo inositol at 0.1 g/l. The media was also supplemented with different concentrations of BAP and IAA as shown in (Table 1) and solidified with 4.5 g/l of agar. The pH was adjusted to 5.8 prior to autoclaving at 121°C for 15 min.