2. Materials and methods
2.1. Microbial cultures
This feasibility study used indigenous pollutant-degrading
bacteria isolated from Cross-Strait Taiwan and Mainland China [13].
All strains were cultured in LB broth medium for 12–16 h and then
the cultures were mixed with 40% (v/v) glycerol to store at
−80 ◦C
for long-term use.