From the Petri dishes, were previously fungal
endophytes were isolated; a 0.5 x 0.5 cm piece
of inoculum was taken and placed on an Erlenmeyer
containing liquid potato dextrose.
Then it was placed on a shaker at 120 rpm at
room temperature in the dark. After eight
days of culture, with a home mixer disinfected
with 70% alcohol for 1 h and 4% sodium hypochlorite
for 30 min, fungi was liquefied till
getting fine particles. 250 ml of liquefied were
obtained and volume was completed till 600
ml on a plastic container.