Regeneration of safflower through tissue culture has long been limited to low frequency and lack of an efficient protocol suitable for most safflower cultivars, which seriously restrict the development of further genetic modification. To solve those problems, scientists had taken lots of measures in experiments, such as select proper explants, change the constituent of culture medium and adjust the culture condition. Excitingly, the rate of successful regeneration increased from approximately 30% to about 90% for some genotypes.