After 72 h incubation, cell
viability was measured using Alamar Blue reagent (Invitrogen Ab,
Lidingö, Sweden) according to the manufacturer’s instructions.
Absorbance was measured at 570 nm with 600 nm as a reference
wavelength. Results were expressed as the mean ± standard error for
six replicates as a percentage of vehicle control (taken as 100%).
Experiments were performed independently at least six times.
Statistical analyses were performed using a two-tailed Student’s t
test. P < 0.05 was considered to be statistically significant