The study of jaguar in Mexico (Rodríguez-Soto et al. 2011) suggests the necessity of generating corridors and offering national spatial strategies for jaguar conservation. Jaguar, as other carnivores, presents low densities and requires extensive habitat areas in order to survive; two of its main threats are habitat fragmentation and demographic isolation (Carroll et al. 2001; Crooks 2002;Singleton et al. 2002). Due to this, identifying the habitat corridors is important for the viability of the species populations. The results of this study can produce guidelines to take action on the protected sites to connect jaguar conservation areas (Beier et al., 2006;Theobald 2006). The goal of this study is to identify the corridors between jaguar conservation and management areas (JCMA) in Mexico (Rodríguez-Soto et al. 2011) and to analyze the viability of the corridors, considering human activities (roads, human settlements, agriculture and livestock rearing). In addition, in two areas of potential corridors we identify “stepping stones” that can help the jaguar movement between large fragments.