Ten transcripts were synthesized using these cDNA templates and 1% denaturing MOPS agarose gel analysis demonstrated that each transcript had the correct molecular size (Figure 1A). Subsequently, approximately equimolar amounts of S1 to S10 cDNA template were mixed and RNA transcripts were synthesized simultaneously in a single reaction (co-synthesis). The in vitro co-transcription products of the EHDV-7 segments showed that ssRNA bands were synthesized in correct sizes similar to the authentic transcripts derived from wild type (WT) viral core (Figure 1B)