2.6.8. Determination of total phenolic content of H. erinaceus
extract, wine and vinegar
The total phenolic content was determined using Folin-
Ciocalteu reagent in accordance with the method of Velioglu,
Mazza, Gao, and Oomah (1998). Folin-Ciocalteu reagent was
diluted 2-fold in deionized water. The sample (0.25 ml) was mixed
with 0.75 ml of diluted FolineCiocalteu reagent and 2ml of Na2CO3
(2.0%, w/v), and then incubated at 36 C for 30 min. Absorbancewas
measured at 750 nm with a UVeVIS spectrophotometer. The concentration
of total phenols was expressed as mg ml1 of extract.