Pernambuco State, Brazil, 1.0% of motor oil, and cell-free
broth solutions (crude biosurfactant) at concentrations of 0.0125%
(at 1/2 CMC), 0.025% (at CMC) and 0.125% (at 5× CMC). Control
flasks contained seawater and motor oil. The flasks were incubated
at 28 ◦C on an orbital shaker rotating at 150 rpm. Triplicate shake
flasks were sacrificed on days 1, 5, 12, 20 and 30 of incubation and
then analyzed for the number of microorganisms by using the most
probable number (MPN)