Pollen collection and culture
Pollen was collected and cultured according to the method
described earlier (Singh et al., 1992). Five replicates were used
for each medium combinationutreatment. All observations on
pollen germination and pollen tube growth were taken 3 h after
incubation. At least 250 pollen was counted from 5±10 random
®elds. A pollen grain was considered germinated if pollen tube
length was greater than the diameter of the pollen grain.